Job Opportunity: Postdoctoral fellowship in pulmonary vascular cell and molecular biology

Posted: January 21, 2016

A post-doctoral research fellowship position is immediately available in the Roberts Lab in the Cardiovascular Research Center (CVRC) at the Massachusetts General Hospital / Harvard Medical School. We investigate novel mechanisms and therapies for pulmonary vascular disease, especially in the developing lung. Using a variety of molecular biology, proteomic, and molecular imaging techniques we have identified new signaling mechanisms that regulate pulmonary vascular smooth muscle cell (SMC) differentiation and proliferation. In particular, we discovered new mechanisms that down-regulate nitric oxide and cGMP signaling in pulmonary and systemic vascular SMC and in animal models of pulmonary vascular disease. A complete list of publications from the Roberts Lab can be viewed at: http://bit.ly/1LjJ2Ra

The goals of the current research program are:
1. To identify molecular mechanisms through which transforming growth factor-beta (TGF-b) regulates nitric oxide and cGMP signaling.

2. To examine how the interplay between TGF-b and NO signaling regulates pulmonary and systemic vascular SMC differentiation and vascular development.

3. To develop novel therapeutic approaches, based on insights derived above, to prevent or ameliorate pulmonary vascular disease.

We are seeking applications from highly motivated candidates with a recent PhD and/or MD, a background in cellular signaling, and evidence of devotion to academics. Candidates for this position should send a curriculum vitae, copies of recent papers that they authored, a brief statement of goals, and three references (names, phone numbers, and email addresses) to: jrobertsjr@mgh.harvard.edu.

Job responsibilities, include:
• With the aid of the PI, refine hypotheses and design experiments related to the goals outlined above.
• Perform technical assays such as immunoblotting and precipitation, epifluorescence microscopy, flow cytometry.
• Perform recombinant DNA/RNA procedures such as plasmid construction, cDNA subcloning, isolation of RNA, and RT-PCR.
• Maintain cell line and primary vascular cell cultures and frozen cell stocks.
• Perform transcription analysis, for example using promoter activation reporter systems
• Conduct literature reviews related to optimizing laboratory methods and preparing manuscripts.
• Write manuscripts and prepare and present research work at meetings.
• Help supervise doctoral and summer students.

Qualifications:
• Recent PhD or MD/PhD with a strong interest in vascular biology.
• Strong background in general molecular and cell biology methods required.
• Experience in examining intracellular signaling, considered a plus.
• Exhibit strong initiative and excellent multitasking skills.
• Ability to lead projects with minimum supervision.
• US citizenship or eligibility for US student visa.

Skills and competencies required:
• Excellent written and verbal communication skills.
• Excellent organizational skills.
• Ability to work productively within a small, friendly, and supportive research group.

Work location and environment:
• Massachusetts General Hospital research facility in the Charlestown Navy Yard-USA.

Overview of the research environment:
The Cardiovascular Research Center at the Massachusetts General Hospital (MGH) is dedicated to investigating the mechanisms of and discovering new therapies for cardiovascular disease. The center occupies over 30,000 sq. ft. of laboratory space and is spread over two campuses: the Charlestown Navy Yard research center, where the Roberts Lab resides, and the Charles River Plaza research facility.  The MGH is a leader in biomedical research and patient care. The MGH is a teaching hospital of Harvard Medical School and the faculty and eligible students also have appointments at that institution. The MGH consistently ranks amongst the medical institutions with the highest funding level of biomedical research and that provide superlative and advanced patient care.